Journal: Cells
Article Title: Extracellular Lactic Acidosis of the Tumor Microenvironment Drives Adipocyte-to-Myofibroblast Transition Fueling the Generation of Cancer-Associated Fibroblasts
doi: 10.3390/cells12060939
Figure Lengend Snippet: Quantitative real-time PCR analysis for the expression of the fibroblast activation/myofibroblast genes FAP ( A ), ACTA2 ( B ), COL1A1 ( C ), and COL1A2 ( D ) in normal human dermal fibroblasts treated for 24 h with conditioned media produced by adipocyte-committed adipose-derived stem cells (acADSCs) grown either under basal (pH 7.4) conditions (basal acADSC-cm) or under lactic acidosis (acidic acADSC-cm). The basal level of each gene expression was set to 1, and the other results are normalized to this value. 18S ribosomal RNA was used as the reference gene. Bars represent the mean ± SEM of three independent experiments ( n = 3 replicates each) from three cell lines. Values of p were determined by the unpaired Student’s t -test.
Article Snippet: Three lines of normal human subcutaneous adipose-derived stem cells (ADSCs) purchased from Lonza (catalog no. PT-5006; Lonza, Basel, Switzerland) were maintained in ADSC complete medium (ADSC Growth Medium BulletKit; catalog no. PT-4505; Lonza) at 37 °C in a 5% CO 2 incubator.
Techniques: Real-time Polymerase Chain Reaction, Expressing, Activation Assay, Produced, Derivative Assay